Figure 10.

Western blot analysis of CcAdoMetS protein expression after treating with 5-azacytidine and L-ethionine. (A) Western blotting showing protein expression level of C. cohnii on different days without any treatment; after treating with 200 μM 5Aza and after treating with 1.2 mM L-Eth. The top panel indicated AdoMet synthetase protein expression level and the lower panel indicated α-tubulin protein expression level which was used as a loading control. (B) A graph indicating the relative expression level of the AdoMet synthetase protein in C. cohnii. Intensities of bands were estimated by using the program "ImageJ". Values were normalized to the α-tubulin internal control and expressed relative to the value of control on day 0. The data are the means ± standard deviations (n = 2). Asterisk (*) indicates significant difference between the control on day 0 and sample values with P < 0.05 by Unpaired t test.