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. 2008 Jan 2;3(1):e1391. doi: 10.1371/journal.pone.0001391

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Lee et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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fru¹ and fru³ males showed vigorous intermale courtship in Flypub without ethanol (No EtOH). Upon daily ethanol treatments in 95% Flypub, their courtship activities did not change with additional ethanol treatment while the courtship levels under ethanol were lower than those without ethanol (ANOVA: fru¹, F_3,27 = 3.24, p = 0.0396, Tukey-Kramer showed a significant difference between No EtOH and 3^rd EtOH; fru³, F_3,27 = 16.94, p<0.0001, No EtOH was significantly different from the others by Tukey-Kramer; n = 7). NS, not significant. CS males tested together with fru males as a control showed negligible intermale courtship in the absence of ethanol and on the 1^st ethanol exposure (marked by diamonds); however, their courtship activities were enhanced under the influence of ethanol on the 2^nd and 3^rd ethanol treatments (F_3,27 = 42.86, p<0.0001; double asterisks, significant difference by Tukey-Kramer).