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. 2008 Jan 2;3(1):e1391. doi: 10.1371/journal.pone.0001391

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Lee et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) TH-GAL4/UAS-Shi^ts and DDC-GAL4/UAS-Shi^ts males, when subjected to daily ethanol exposure at 32°C to block synaptic output of dopamine neurons, displayed drastically reduced intermale courtship compared to the control mw⁺/UAS-Shi^ts males on all exposures (1^st exposure, F_2,21 = 18.24, p<0.0001; 2^nd, F_2,21 = 35.26, p<0.0001; 3^rd, F_2,21 = 27.02, p<0.0001; n = 7–8). Double asterisks, significant difference by Tukey-Kramer tests. (B) TH-GAL4/UAS-Shi^ts males, when subjected to daily ethanol exposure at room temperature, showed intermale courtship activities comparable to those of the control TH-GAL4/UAS-GFP males, indicating that Shi^ts expressed in dopamine neurons without the dominant negative activity has no effect on the ethanol-induced intermale courtship (Student t-test on all exposures, p>0.5, n = 7).