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Both the V and C domain of the HOPC 2020 λ LC possess potential BiP-binding sites. The BiP score algorithm was applied to the amino acid sequence and predicts a value for each eight amino acids. The value is assigned to the first amino acid of the octapeptide. Values of between six and 10 are predictive of possible BiP-binding sites and values above 10 are considered highly predictive. Negative values identify sequences that are very unlikely to stimulate the ATPase activity of BiP.
