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. 1999 Jan 11;144(1):45–57. doi: 10.1083/jcb.144.1.45

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Distribution of d-pinch RNA during Drosophila development. (A) Lateral view of a stage 9 embryo, hybridized with antisense d-pinch probe; oriented with anterior left, dorsal side up. The transcript is concentrated in the developing mesoderm, whereas there is no specific staining seen with the negative control (data not shown). (B) Dorsal view of an early stage 13 embryo, hybridized with antisense d-pinch probe. D-pinch transcript is detected strongly in the visceral mesoderm flanking the developing gut (arrowheads), as well as in the somatic musculature. (C) Dorsal view of a late stage 16 embryo, hybridized with antisense d-pinch probe. The d-pinch transcript is still strongly expressed in the visceral mesoderm (white arrowheads) that now completely envelopes the gut and the somatic body wall muscles (black arrowheads). Pharyngeal muscle and epidermal tendon cell staining is also present at this stage; however, these tissues are not discernible in this plane of focus. (D) Isolated epidermis from a stage 16 embryo, hybridized with d-pinch probe. Note the strong hybridization at the segment boundaries where the somatic muscle cells attach to the epidermis. Arrowheads, two attachment sites.