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. 1998 Nov 2;143(3):795–813. doi: 10.1083/jcb.143.3.795

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Distribution of paralemmin among subcellular fractions of the purification course of chicken synaptic plasma membranes, in comparison to GAP-43 and amphiphysin. 25 μg of protein from each fraction was analyzed by immunoblotting. Fraction designations are as in Babitch et al. (1976). P₂′″, washed 13,600 rpm pellet of brain homogenate (“mitochondrial pellet”). Fractions and subfractions of P₂′″ after Ficoll step gradient: F_m, “mitochondrial fraction;” S_cm and P_cm, “myelin supernatant and pellet;” S_s and P_s, “synaptosomal supernatant and pellet.” Fractions and subfractions after hypotonic lysis of P_s synaptosomes: P_ls, “intrasynaptosomal mitochondria;” P_hs, large membranes; S_hs, cytosol and small vesicles. Saccharose step gradient fractions of P_hs: 0/0.4, interface between 0 and 0.4 M saccharose, etc.; P, mitochondria-rich pellet. The course of purification of synaptic plasma membranes is P₂′″–P_s–P_hs; 0.4/0.6/0.8.