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. 1998 Nov 2;143(3):719–736. doi: 10.1083/jcb.143.3.719

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Western blot analysis of Gin4p. Proteins from exponential-phase cells of the indicated strains were stained with antibodies to Gin4p (top) and to Cdc11p (bottom; to confirm equal protein loading). Lanes 1 and 2, strain YEF473 (wild-type) containing no plasmid (lane 1) or plasmid YEp/GIN4 (lane 2); lanes 3–6, strain M-272 (gin4-Δ9/gin4-Δ9) containing no plasmid (lane 3), plasmid YCp/GIN4 (lane 4), plasmid YCp/GIN4-3HA (lane 5), or plasmid YCp/gin4^K48M (lane 6). Sizes of molecular weight markers (in kD) are indicated.