Table II.
Plasmids Used in This Study
| Name | Description* | Source | ||
|---|---|---|---|---|
| YCplac111 | CEN, LEU2 | Gietz and Sugino, 1988 | ||
| YEplac181 | 2 μ, LEU2 | Gietz and Sugino, 1988 | ||
| YIplac204 | URA3 | Gietz and Sugino, 1988 | ||
| pEG202 | 2 μ, HIS3, lexA DNA-binding domain (DBD) | Ausubel et al., 1995 | ||
| pJG4-5PL | 2 μ, TRP1, transcriptional activation domain (AD) | DeMarini et al., 1997 | ||
| pB12G | 2 μ, LEU2, CDC12, ADE3 | Fares et al.2 | ||
| YEp24(CDC12)N | 2 μ, URA3, CDC12 | Fares et al.2 | ||
| YCp50[MPK1] | CEN, LEU2, MPK1 | Lee et al., 1993 | ||
| YCpGALGST | CEN, LEU2, GST under GAL1/10 promoter control | See text | ||
| YCp/CDC12 | CEN, LEU2, CDC12 | See text | ||
| P54-6 | CEN, LEU2, ∼12 kb of chromosome VIII DNA including MPK1 (SLT2) | See text | ||
| p54-15 | CEN, LEU2, ∼12 kb of chromosome IV DNA including GIN4 | See text | ||
| YCp/GIN4 | CEN, LEU2, GIN4 | See text and Fig. 1 | ||
| YEp/GIN4 | 2 μ, LEU2, GIN4 | See text | ||
| pDK49 | URA3, gin4K48A | Altman and Kellogg, 1997 | ||
| pDK50 | CEN, URA3, gin4K48A | D. Kellogg, University of California, Santa Cruz, CA | ||
| YCp/gin4K48M | CEN, LEU2, gin4K48M | See text | ||
| YEp/gin4K48M | 2 μ, LEU2, gin4K48M | See text | ||
| YCp/GIN4-3HA | CEN, LEU2, GIN4-3HA | See text | ||
| YCp/gin4K48M-3HA | CEN, LEU2, gin4K48M-3HA | See text | ||
| YIp/GIN4-GST | URA3, GIN4-GST | See text | ||
| YCpGALGST/GIN4N | CEN, LEU2, GAL1/10-GST-GIN4 (codons 1–315) | See text | ||
| YCpGALGST/GIN4C | CEN, LEU2, GAL1/10-GST-GIN4 (codons 295–1,142) | See text | ||
| pEG202/GIN4N | 2 μ, HIS3, DBD-GIN4 (codons 1–294) | See text | ||
| pEG202/GIN4C | 2 μ, HIS3, DBD-GIN4 (codons 295–1,142) | See text | ||
| pEG202/GIN4NFL | 2 μ, HIS3, DBD-GIN4 (codons 18–1,142) | See text |
*
CEN plasmids are low copy; 2 μ plasmids are high copy; other plasmids do not have a yeast replication origin and can transform only by chromosomal integration.