Table VI.

Chitin Localization in Wild-type and gin4-Δ9 Strains

Strain		Temperature		Chitin localization
				Asymmetric		Symmetric		Delocalized
		(°C)		(%)		(%)		(%)
YEF473		30		100		0		0
M-272		23		13		87		0
		30		4		96		0
		37		2		19		79

Strains YEF743 (wild-type) and M-272 (gin4-Δ9/gin4-Δ9) were grown to exponential phase at the temperature indicated and stained with Calcofluor. For each sample, 100 budded cells were scored for their patterns of chitin staining. Asymmetric, chitin localized to the mother side of the mother–bud neck (see Fig. 9 G for examples). Symmetric, chitin localized to the neck region but with apparently equal staining intensity on both mother and bud sides of the neck (see Fig. 9 H for examples). Delocalized, chitin dispersed more or less uniformly around the cell periphery. Chitin localization in YEF473 cells grown at 23° or 37°C was indistinguishable from that of YEF473 cells grown at 30°C.