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. 1998 Nov 2;143(3):751–765. doi: 10.1083/jcb.143.3.751

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CDC31 and KIC1 interact in vivo. (A) cdc31-1 kic1-1 double mutants have a synthetic lysis defect. The cdc31-1 kic1-1 strain (MY4977) was incubated in YPD at 37°C for 8 h in the presence (+) or absence (−) of 1 M sorbitol and analyzed microscopically. Asterisks, lysed cells. (B) The elongated bud defect of Kic2p overexpression is suppressed by increasing the expression of Cdc31p. Overexpression of the Kic1p (Kic1p O/E) protein induces elongated bud growth (−CDC31 2μ, strain MY4123) that is suppressed when CDC31 is expressed off a high copy vector (+CDC31 2μ, strain MY5493).