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. 1998 Nov 2;143(3):563–575. doi: 10.1083/jcb.143.3.563

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The immunofluorescence staining corresponding to the Triton-resistant fraction of the p150 and p60 subunits disappears following DNase I treatment. Asynchronous cells were extracted with Triton in the absence (without DNase I) or in the presence of 0.1 mg/ml DNase I (with DNase I). After fixation, cells were stained with a monoclonal anti-p150 antibody (p150) and polyclonal anti-p60 antibody (p60*). DAPI staining (DNA) confirmed the disappearance of DNA after DNase I digestion. Monoclonal antinucleoporins antibody (pore) was used to verify the integrity of the nuclear envelope. Bar, 10 μm.