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. 1998 Nov 2;143(3):601–612. doi: 10.1083/jcb.143.3.601

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Comparison of effects of antibodies directed against the COOH termini of p23 and p24 on retrograde transport of CTX-A–K63 from the Golgi to the ER. (a and b) Vero cells that were neither treated with CTX– K63 nor microinjected, were fixed, permeabilized, and then immunostained for p24 or p23 using Cy5- and Cy2- labeled primary antibodies, respectively. Both antibodies stain similar structures, mainly representing Golgi membranes. (c and d) Vero cells were loaded with CTX–K63 as given in Fig. 2. About 20 min after the start of CTX– K63 uptake, antibodies against p24 were microinjected. As control, cells from the same coverslip were injected with antibodies directed against the COOH terminus of p23 (inset). Cells were then incubated for another 80 min, fixed, and then analyzed for the distribution of CTX-A–K63 (c) or p24 (d) or p23 (inset in d) by laser scan immunofluorescence microscopy. The cell microinjected with the anti-p24 antibodies is marked by an asterisk. Bar, 10 μm.