Figure 4.

Uptake of CTX–K63 leads to a concomitant dispersion of Erd2p and p53 in Vero cells. Vero cells were incubated with 0.5 μg/ml CTX–K63 and the uptake started as in Fig. 2. At zero time (top panels) and after 50 min (bottom panels) cells were fixed and immunostained for Erd2p and p53 using Cy2-labeled anti-Erd2p (rabbit) and a mouse anti-p53 antibody. Note that Erd2p as well as p53 change from a cap-like to a more circular distribution in the presence of the KDEL cargo (CTX-A–K63). Bar, 10 μm.