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. 1998 Nov 2;143(3):601–612. doi: 10.1083/jcb.143.3.601

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F_ab fragments directed against the cytoplasmic tail of Erd2p inhibit retrograde transport of CTX-A–K63 from the Golgi to the ER in Vero cells. The F_ab fragments were microinjected 10–15 min before uptake of CTX– K63 was initiated as given in the Materials and Methods section. The cells were further incubated for 120 min. The cells were then fixed and immunostained for CTX-A (a and c). The microinjected cells (a and b, arrows) were identified by the immunofluorescence of Cy2-labeled anti–Erd2p F_ab fragments that had been mixed at a 1:3 ratio with non-labeled anti– Erd2p F_ab fragments (b and d). Note the retention of CTX-A–K63 in perinuclear structures in cells microinjected with the anti–Erd2p F_ab fragments. a and b show the results of laser scan microscopy; c and d show the results of conventional immunofluorescence microscopy. Bar, 10 μm.