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. 1998 Nov 2;143(3):687–694. doi: 10.1083/jcb.143.3.687

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Motor mutants delay anaphase onset. cin8Δ causes a delay in the entry into anaphase. Strains AFS501 (WT), AFS426 (cin8Δ), AFS404 (kip1Δ), and AFS417 (kip3Δ) were synchronized in G1 by treatment with 10 μg/ml α-factor. Cells were released from the block and were assayed for sister chromatid separation at 15 minute intervals as previously described (Straight et al., 1996). The percentage of cells with separated sister chromatids versus time is shown.