Table I.
Yeast Strains
| Strain number | Relevant genotype | Plasmid | ||
|---|---|---|---|---|
| AFS34 (W303-1a) | MATa ade2-1 can1-100 ura3-1 leu2-3,112 his3-11,15 trp1-1 | |||
| AFS403 | MATa his3-11,15::GFP-LacI-HIS3 leu2-3,112::lacO-LEU2 ura3-1::GFP-TUB1-URA3 | pAFS78 | ||
| pAFS59 | ||||
| pAFS91 | ||||
| AFS426 | MATa cin8Δ::TRP1 his3-11,15::GFP-LacI-HIS3 leu2-3,112::lacO-LEU2 ura3-1::GFP-TUB1-URA3 | " | ||
| AFS404 | MATa kip1Δ::HIS3 his3-11,15::GFP-LacI-HIS3 leu2-3,112::lacO-LEU2 ura3-1::GFP-TUB1-URA3 | " | ||
| AFS417 | MATa kip3Δ::HIS3 his3-11,15::GFP-LacI-HIS3 leu2-3,112::lacO-LEU2 ura3-1::GFP-TUB1-URA3 | " | ||
| AFS501 | MATa his3-11,15::GFP13-LacI-I12-HIS3 leu2-3,112::lacO-LEU2 ura3-1::GFP-TUB1-URA3 | pAFS144 | ||
| pAFS59 | ||||
| pAFS91 |
All strains are isogenic to AFS34 (W303-1a from the laboratory of R. Rothstein). Only the genotype that differs from AFS34 is shown.