Table II.
Spindle Dynamics in Wild-type and Kinesin Mutants
| Strain | Fast phase | Slow phase | Breakdown length | Anaphase duration | Anaphase A | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| μm/min | μm/min | μm | min | |||||||
| WT | 0.54 ± 0.02 | 0.21 ± 0.01 | 9.52 ± 0.49 | 26.6 ± 1.6 | 1.33 μm/min | |||||
| cin8Δ | 0.28 ± 0.02 | 0.19 ± 0.02 | 6.95 ± 0.68 | 26.7 ± 2.4 | ND | |||||
| kip1Δ | 0.51 ± 0.03 | 0.12 ± 0.03 | 9.79 ± 0.43 | 43.0 ± 3.1 | ND | |||||
| kip3Δ | 0.56 ± 0.04 | 0.17 ± 0.02 | 12.31 ± 0.25 | 38.5 ± 3.1 | 1.30 μm/min |
Rates of spindle elongation were calculated using linear regression analysis in the same time intervals used to calculate the wild-type rates for the fast and slow phases of anaphase. Measurements shown in bold are significantly different from wild-type rates and all rates are displayed as the mean ± SE. The average breakdown length and the average duration of anaphase were calculated using the final timepoint before spindle disassembly. The total number of records analyzed for measurements of WT, cin8Δ, kip1Δ, and kip3Δ are n = 6, n = 5, n = 5, and n = 7, respectively.