Table II.
Osmolarity of Buffers Used in ATP Efflux Assay
Buffer base | Predominant anion composition | Osmolarity | ||
---|---|---|---|---|
mOsmol | ||||
HPBR | 140 mM NaCl | 303 ± 5 | ||
140 mM Na-gluconate | 295 ± 3 | |||
140 mM NaBr | 293 ± 4 | |||
SOS | 100 mM NaCl | 205 ± 2 | ||
100 mM Na-gluconate | 204 ± 2 | |||
100 mM NaBr | 204 ± 3 |
The osmolarity of these solutions was determined on a Multi-Osmette Model 2430 Automatic Osmometer (Precision Systems, Natick, MA).