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. 1998 Nov 2;143(3):837–847. doi: 10.1083/jcb.143.3.837

Figure 4.

Figure 4

Figure 4

Figure 4

HA decreases CD44 surface expression. (A) Rat alveolar macrophages were isolated, plated at 5 × 106 cells/ml in quadruplicate wells, using 96-well dishes (5 × 104 cells/well), and cultured in fusogenic conditions for 2 d, in the absence or presence of 1 mg/ml BSA, chondroitin sulfate A (CSA) or B (CSB), hyaluronic acid (HA), or 1 μM osteopontin (OP). CD44 expression was determined by ELISA using mAb mouse anti–rat CD44 followed by goat anti–mouse IgG-conjugated to horseradish peroxidase. (B) Cells were cultured as in A in the absence or presence of decreasing concentrations of HA and CD44 expression was determined by ELISA. (C) Cells were cultured as in A in the absence or presence of either OP (1 μM) or HA (1 mg/ml) for 3 d. CD44 expression was determined by ELISA in which mAb anti-CD44 incubation buffer was supplemented or not with either OP (1 μM) or HA (1 mg/ml).