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Inhibition of the proteasome by either LLnL or lactacystin prolonged serum-induced MAPK nuclear translocation. Parental CCL39 cells were grown on glass coverslides, and p42/p44 MAPK immunolabeling was performed as described in Fig. 1, except that before fixation (A) cells were stimulated 9 h with 10% FCS; (B) cells were stimulated for 9 h with 10% FCS in the presence of 30 μM of LLnL; (C) cells were stimulated for 9 h with 10% FCS in the presence of 10 μM lactacystin. Bar, 10 μm.
