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. 1998 Aug 10;142(3):827–835. doi: 10.1083/jcb.142.3.827

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The effect of forskolin on microphthalmia promoter activity is mediated by a consensus CRE motif (−147/−140). B16 cells were transfected with 0.45 μg of pMI, pMIΔ1, pMIΔ2, pMIΔ3, pMIΔ4, pMIΔ5, or pMImCRE and 0.05 μg of pCMVβGAL. After 12 h with forskolin, luciferase activity was normalized by the β-galactosidase activity and the results were expressed as fold stimulation of the basal luciferase activity from unstimulated cells. Data are means ± SE of five experiments performed in triplicate. ▿ indicates the TATA box position.