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. 1998 Aug 10;142(3):827–835. doi: 10.1083/jcb.142.3.827

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The CRE motif do not confer cAMP sensitivity to microphthalmia promoter in NIH3T3 fibroblast cells. NIH3T3 fibroblast cells were transfected with 0.45 μg of pMI, pMIΔ1, pMIΔ2, pMIΔ3, pMIΔ4, pMIΔ5, pMImCRE, or CRE-LUC and 0.05 μg of pCMVβGAL. After 12 h with forskolin, luciferase activity was normalized by the β-galactosidase activity and the results were expressed as fold stimulation of the basal luciferase activity from unstimulated cells. Data are means ± SE of five experiments performed in triplicate. ▿ indicates the TATA box position.