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. 1998 Aug 10;142(3):827–835. doi: 10.1083/jcb.142.3.827

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Regulation of tyrosinase promoter activity by forskolin is mediated by microphthalmia. B16 cells were transfected with (A) 0.4 μg of pTyro or (B) 0.4 μg of a CRE-LUC reporter plasmid, 0.05 μg of pCMVβGAL, and different amounts of pCDNA3 encoding Mi-ΔNT. After 24 h with forskolin, luciferase activity was normalized by the β-galactosidase activity and the results were expressed as fold stimulation of the basal luciferase activity from unstimulated cells. Data are means ± SE of five experiments performed in triplicate.