Table II.
Yeast Strains
| Strain | Genotype* | Reference | ||
|---|---|---|---|---|
| CRY1 | MAT a ade2-1oc can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | R. Fuller (Stanford University, CA) | ||
| JGY46 | MAT a/MATα ade2-1oc/ade2-1oc can1-100/can1-100 his3- | (Geiser et al., 1991) | ||
| 11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 | ||||
| EMY55-5D | MAT a ade2-1oc ade3Δ-100 can1-100 cyh2r his3-11,15 leu2-3,112 lys2Δ::HIS3 trp1-1 ura3-1 | E. Muller (University of | ||
| Washington, Seattle, WA) | ||||
| EMY55-6B | MATα ade2-1oc ade3Δ-100 can1-100 cyh2S his3-11,15 leu2-3,112 lys2Δ::HIS3 trp1-1 ura3-1 | E. Muller (University of | ||
| Washington) | ||||
| MMY28 | MAT a ade2-1oc ade3Δ-100 can1-100 (S65T-GFP)–CMD1 his3- | (Moser et al., 1997) | ||
| 11,15 leu2-3,112 lys2Δ::HIS3 trp1-1 ura3-1 | ||||
| RSY1 | EMY55-5D X EMY55-6B | This study | ||
| RSY2 | MAT a /MATα ade2-1oc/ade2-1oc ade3Δ-100/ade3Δ-100 can1-100/can1-100 cyh2r/cyh2S | This study | ||
| his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 lys2Δ::HIS3/lys2Δ::HIS3 MYO2/myo2Δ::TRP1 | ||||
| trp1-1/-trp1-1 ura3-1/ura3-1 | ||||
| RSY2-60B | MAT a ade2-1oc ade3 Δ-100 can1-100 his3-11,15 leu2-3,112 | This study | ||
| lys2Δ::HIS3 myo2Δ::TRP1 trp1-1 ura3-1 carrying plasmid pRS50 | ||||
| RSY21 | MAT a ade2-1oc can1-100 his3-11,15 leu2-3,112 MYO2-D6IQ trp1-1 ura3-1 | This study | ||
| RSY22 | MATα ade2-1oc can1-100 his3-11,15 leu2-3,112 MYO2-D6IQ trp1-1 ura3-1 | This study | ||
| RSY38 | MAT a /MATα ade2-1oc/ade2-1oc ADE3/ade3Δ-100 can1-100/can1-100 CMD1/GFP–CMD1 | This study | ||
| his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 MYO2/MYO2-Δ6IQ MYO4/myo4Δ::URA3 | ||||
| trp1-1/trp1-1 ura3-1/ura3-1 | ||||
| RSY38-9D | MAT a ade2-1oc ade3Δ-100 can1-100 GFP–CMD1 his3-11,15 | This study | ||
| leu2-3112 lys2Δ::HIS3 MYO2-Δ6IQ trp1-1 ura3-1 | ||||
| RSY38-16A | MAT a ade2-1oc ade3Δ-100 can1-100 GFP–CMD1 his3-11,15 | This study | ||
| leu2-3,112 lys2Δ::HIS3 myo4Δ::URA3 trp1-1 ura3-1 | ||||
| RSY38-16C | MATα ade2-1oc ade3Δ-100 can1-100 his3-11,15 leu2-3,112 | This study | ||
| lys2Δ::HIS3 MYO2-Δ6IQ myo4Δ::URA3 trp1-1 ura3-1 | ||||
| RSY38-17C | MATα ade2-1oc ade3Δ-100 can1-100 GFP–CMD1 his3-11,15 | This study | ||
| leu2-3,112 lys2Δ::HIS3 trp1-1 ura3-1 | ||||
| RSY105 | MAT a/MATα ade2-1 oc/ade2-1 oc can1-100/can1-100 his3- | This study | ||
| 11,15/his3-11,15 leu2-3,112/leu2-3,112 MLC1/mcl1D::TRP1 | ||||
| trp1-1/trp1-1 ura3-1/ura3-1 carrying plasmid pRS289 | ||||
| RSY105-6A | MATα ade2-1oc can1-100 his3-11,15 leu2-3,112 mcl1Δ::TRP1 | This study | ||
| trp1-1 ura3-1 carrying plasmid pRS289 | ||||
| RSY107 | RSY21 X RSY105-6A carrying plasmid pRS289 | This study | ||
| RSY112 | MAT a/MATα ade2-1oc/ade2-1oc ade3Δ-100/ade3Δ-100 can1-100/can1-100 his3-11,15/his3-11,15 | This study | ||
| leu2-3,112/leu2-3,112 lys2Δ::HIS3/lys2Δ::HIS3 MLC1/mcl1Δ::TRP1 | ||||
| MYO2/myo2Δ::TRP1 trp1-1/trp1-1 ura3-1/ura3-1 carrying plasmid pRS289 |
*
GFP–CMD1 encodes a fusion protein of green fluorescent protein and calmodulin.