Table II.
Frequencies of Meiotic Figures Observed in asl1 and Control (Oregon R) Testes
| Genotype | Total cells scored | Phases and stages of meiosis | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| L. Prophase I E. Prometa. I (M1a, b) | Prometa. I Metaphase I (M2; M3) | E. Anaphase I (M4a) | Anaphase I Telophase I (M4b,c; M5) | Interphase II Prometa. II Metaphase II E. Anaphase II (M6; M7; M8; M9; M10a) | Anaphase II Telophase II (M10b,c; M11) | |||||||||
| % | % | % | % | % | % | |||||||||
| asl1/asl 1 | 820 | 21.6 | 19.0 | 13.0 | 23.2 | 11.1 | 12.1 | |||||||
| Oregon R | 1,058 | 14.8 | 5.8 | 0.9 | 19.5 | 31.8 | 27.2 | |||||||
| Ratios | 1.5 | 3.3 | 14.4 | 1.2 | 0.3 | 0.4 | ||||||||
The stages of wild-type meiosis are described in detail by Cenci et al. (1994; see also Figs. 2, 4, and 5). Some of the equivalent stages observed in asl1 mutants are shown in Figs. 3, 4, 5, and 7. For example, the meiotic figures in Fig. 3, A, B, C, D–F, and G correspond to the M1, M2, M3, M4a, and M5 stages of the wild type, respectively. In asl mutants, early anaphase II is difficult to distinguish from metaphase II because in both types of cells some chromosomes exhibit sister chromatid separation. Therefore, these stages have been grouped together. Ratios are between the frequencies of meiotic figures observed in asl and in control. The numbers of meiotic figures have been determined by examining 11 Oregon R testes and 10 asl1 testes. E, early; L, late; Prometa, prometaphase.