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. 1998 Aug 10;142(3):651–663. doi: 10.1083/jcb.142.3.651

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Kinetics of processing of ALP, (F/A)A-ALP, and (F/A) RS-ALP in wild-type and apm3Δ cells. SNY17 (wild-type) and NBY100 (apm3::HIS3) pho8Δ-X PEP4 cells harboring pSN92 (ALP), pSN55 (A-ALP), or pSN123 ((F/A)RS-ALP) were labeled with [³⁵S]Met for 10 min and chased by adding unlabeled methionine and cysteine each to a final concentration of 50 μg/ml. At the indicated times, proteins were immunoprecipitated from cell extracts using polyclonal antibodies against ALP. The resulting immunoprecipitates were subjected to SDS-PAGE and fluorography.