Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Aug 10;142(3):651–663. doi: 10.1083/jcb.142.3.651

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

RS-ALP is mislocalized to the vacuolar membrane of vac7 mutant cells. (A) NBY85 (vac7-1 pho8Δ-X pep4Δ-X) cells harboring pSN92 (ALP), (B) pSN97 (RS-ALP), and (C) pSN55 (A-ALP) were prepared for double labeling indirect immunofluorescence using the α-ALP mAb 1D3-A10, and affinity-purified antibodies against the 100-kD subunit of the V-ATPase, Vph1p, as described in Materials and Methods. Cells were also visualized using DIC microscopy (k–o). (D) NBY85 (vac7-1 pho8Δ-X pep4Δ-X) cells harboring pSN97 (RS-ALP) were prepared for double labeling indirect immunofluorescence using the α-ALP mAb 1D3-A10 and affinity-purified antibodies against Vps10p, as described in Materials and Methods. Cells were also visualized using DIC microscopy.