Table I.

. Effect of Nocodazole on the Disappearance of C₆-NBD-SM and C₆-NBD-GlcCer from BC

		Percent of NBD-positive BC
		C6-NBD-GlcCer		C6-NBD-SM
Before chase		100		100
After chase
control		61 ± 5		59 ± 4
nocodazole		58 ± 2		57 ± 3

Cells were incubated with C₆-NBD-GlcCer or -SM at 37°C for 30 min. The fluorescent pool of C₆-NBD-lipids in the basolateral PM was then back exchanged in the absence or presence of nocodazole. Cells were then rewarmed to and further incubated in back exchange medium with or without nocodazole at 18°C for 1 h. The percentage of NBD-positive BC (i.e., BC alone and BC + SAC) was determined as described in Materials and Methods. The percentage of NBD-positive BC before the chase was set at 100%. Data are expressed as mean ± SEM of at least two independent experiments.