Table II.
Sorting of mtDNA Is Altered in Zygotes Lacking Abf2p
| % of total zygotes with a medial bud* | ||||||||
|---|---|---|---|---|---|---|---|---|
|
||||||||
| Time | ||||||||
| (h) | ||||||||
| 2 | 8 | 8 | 36 | 48 | ||||
| 3 | 20 | 2 | 42 | 36 | ||||
| 4 | 30 | 4 | 50 | 16 | ||||
| 5 | 64 | 0 | 30 | 6 | ||||
Synchronized matings were performed using strain S150-2B Δabf2 ρ+ prelabeled with CS1-GFP and strain PSY142 Δabf2 ρ0 cells as described in Materials and Methods. Cells were processed and analyzed as described in Table I.
50 zygotes with one-third to two-thirds the size of a diploid bud were scored for each time point after mating.
