Table II.
Sorting of mtDNA Is Altered in Zygotes Lacking Abf2p
% of total zygotes with a medial bud* | ||||||||
---|---|---|---|---|---|---|---|---|
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Time | ||||||||
(h) | ||||||||
2 | 8 | 8 | 36 | 48 | ||||
3 | 20 | 2 | 42 | 36 | ||||
4 | 30 | 4 | 50 | 16 | ||||
5 | 64 | 0 | 30 | 6 |
Synchronized matings were performed using strain S150-2B Δabf2 ρ+ prelabeled with CS1-GFP and strain PSY142 Δabf2 ρ0 cells as described in Materials and Methods. Cells were processed and analyzed as described in Table I.
50 zygotes with one-third to two-thirds the size of a diploid bud were scored for each time point after mating.