Table I.
Yeast Strains
| Strain name* | Genotype | Derivation | ||
|---|---|---|---|---|
| W303 | MATa/MATα, leu2-3,112/leu2-3,112, his3-11,15/his3-11,15, trp1-1/trp1-1, can1-100/can1-100, ade2-1/ade2-1, ura3-1/ura3-1 | Thomas and Rothstein, 1989 | ||
| CSDC01a/α | W303 Mata/Matα, mlp1::URA3/MLP1 | This study | ||
| CSDS02a | W303 Mata, mlp1::URA3 | Segregant of CSDC01a/α; this study | ||
| CSDC03α | W303 Matα, mlp1::URA3 | Segregant of CSDC01a/α; this study | ||
| CSDC04a/α | W303 Mata/Matα, mlp2::HIS3/MLP2 | This study | ||
| CSDC05a | W303 Mata, mlp2::HIS3 | Segregant of CSDC04a/α; this study | ||
| CSDC06α | W303 Matα, mlp2::HIS3 | Segregant of CSDC04a/α; this study | ||
| CSDC07a/α | W303 Mata/Matα, mlp1::URA3/MLP1, mlp2::HIS3/MLP2 | This study | ||
| CSDC08α, mlp2Δ | W303 Matα, MLP1, mlp2::HIS3 | Segregant of CSDC07a/α; this study | ||
| CSDC09α, mlp1&mlp2Δ | W303 Matα, mlp1::URA3, mlp2::HIS3 | Segregant of CSDC07a/α; this study | ||
| CDSC10a, mlp1Δ | W303 Mata, mlp1::URA3, MLP2 | Segregant of CSDC07a/α; this study | ||
| CSDC11a, wt | W303 Mata, MLP1, MLP2 | Segregant of CSDC07a/α; this study | ||
| CSDC12a | W303 Mata, mlp1::URA3, mlp2::HIS3 | Segregant of CSDC07a/α; this study | ||
| CSDC13a/α, mlp1&mlp2Δhd | W303 Mata/Matα, mlp1::URA3/mlp1::URA3, mlp2::HIS3/mlp2::HIS3 | This study | ||
| CDSC14a/α | W303 Mata/Matα, mlp1-proteinA::HIS3, URA3/MLP1 | This study | ||
| CSCD15a, mlp1-pA | W303 Mata, mlp1-proteinA::HIS3, URA3 | Segregant of CSDC14a/α; this study | ||
| CSDC17a/α | W303 Mata/Matα, mlp2-proteinA::HIS3, URA3/MLP2 | This study | ||
| CSDC18a, mlp2-pA | W303 Mata, mlp2-proteinA::HIS3, URA3 | Segregant of CSDC17a/α; this study | ||
| W303/pGALMLP1 | W303 Mata/Matα, pGALMLP1::URA3 | This study |
*
All strains are isogenic to W303. The most important strains for the experiments described in this paper were given a second, more descriptive name that is used in the text and in the figure legends. This designation is indicated in bold characters.