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. 1999 Mar 8;144(5):869–881. doi: 10.1083/jcb.144.5.869

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Schematic of reagents used in this study. mAbs and pAbs against the cytoplasmic domains of several SNARE proteins were characterized previously. Antibodies against Bip and calnexin were used to identify the ER. The temperature-sensitive form of VSV-G coupled to fluorescent proteins serves as a secretory pathway cargo visible in living cells. B, C, G, and YFP were fused to a variety of SNARE proteins. Coupling to the NH₂-terminal of the SNARE results in a fluorescent protein exposed to the cytoplasm, whereas fusion to the COOH-terminal end places the fluorescent protein within the lumen.