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. 1999 Apr 5;145(1):83–98. doi: 10.1083/jcb.145.1.83

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Transient expression of GFP-tagged COOH-terminal domain of GMAP-210. Cells were transfected with GFP-ΔN1778, processed and labeled with different markers. (a–c) A low magnification image showing a GFP-ΔN1778 expressing cell surrounded by several nontransfected cells (a, green). After transfection, cells were labeled with the centrosomal marker CTR453 (b, red). In c, superposition of both labelings is shown. Note that no staining with CTR453 could be detected in the GFP–COOH-terminal domain expressing cell. a_1–3 (green) show increasing expression levels of GFP-ΔN1778; b_1–3(red) show staining with CTR453 and in c_1–3superpositions of both labelings are shown. (d–f) Effect of overexpression of GFP–COOH-terminal domain on the Golgi apparatus. Image pairs of GFP-ΔN1778 (green) and RM130 (d and e, red) or CTR433 (f, red) are presented. (g–i) GFP-ΔN1778 overexpressing cell (g, green) labeled for detyrosinated tubulin (h, red). Image pair is shown in i. Bars, 10 μm.