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. 2007 Dec 20;118(1):79–88. doi: 10.1172/JCI33700

Figure 3. CQ is a p53-dependent chemoprevention agent.

Figure 3

(A) CQ induces cell death in wild-type, Atm- and Arf-deficient MEFs, but not in p53-deficient MEFs. Early passage (p2) MEFs paired wild-type, Atm–/–, Arf–/–, and p53–/– MEFs were left untreated or were treated with CQ (50 μM) for 24 h. The percentage cell death was determined by staining cells with propidium iodide. Results shown are the mean of 3 independent experiments. (B) Administration of CQ i.p. impairs thymoma development in Atm-deficient mice. Beginning at weaning, Atm–/– mice were treated with 3.5 mg/kg CQ (in PBS) i.p. every 5 days, or with PBS alone (n = 20 for each group). Median survival time was 147 days for mice treated with PBS versus 254 days for mice receiving CQ (P < 0.0001). (C) Administration of CQ i.p. does not affect thymoma development in p53-deficient mice. At weaning, p53–/– mice were treated with 3.5 mg/kg CQ (in PBS) i.p. every 5 days, or with PBS alone (n = 20 for each group). Median survival time was 198 days for mice in both groups.