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. 2007 Dec 14;28(5):798–809. doi: 10.1016/j.molcel.2007.09.020

Figure 2.

Figure 2

Normal Templated and Untemplated Diversification of the Ig Locus in ΔUSP1 Cells

(A) Loss of surface IgM fluctuation analysis in the WT and two clones of ΔUSP1 strains. For each cell line, 24 clones were individually expanded for up to 30 days, after which they were assayed by FACS for loss of sIgM expression. Numbers depict the median value for the prevalence of sIgM-negative cells.

(B) Quantitation of mutational events in sorted sIgM-loss variants. Mutations were expressed as changes per altered sequence that were templated (gene conversion), deletions, or duplications and point mutations (untemplated). In total, 45 different mutated sequences (where clonality was eliminated) were compared to a large existing WT database of 456 sequences.

(C) Gain of sIgM fluctuation analysis of the clone 18 frameshift. In this cell line, gene conversion will restore sIgM expression. Twenty-four clones from the WT (CL 18) and two clones of ΔUSP1 were expanded in culture for 30 days. Surface IgM was determined by FACS analysis, and the indicated number denotes the median value for the prevalence of sIgM expression.