Fig. 2.

Dependence of hypothalamic malonyl-CoA on glucose. (A) Mice deprived of food for 20 h were given 1, 2, 4, and 8 g of glucose or vehicle per kilogram of body weight. Plasma was collected at 30 min after injection, and glucose was determined. Hypothalami were collected at 1 h, and malonyl-CoA concentration was determined (n = 4 per group) (P > 0.002; malonyl-CoA ANOVA). (B) Mice deprived of food for 20 h were fed a high-carbohydrate/low-fat diet and vehicle, a low-carbohydrate/high-fat diet and vehicle, or low-carbohydrate/high-fat diet and 4 g of glucose per kilogram of body weight. Plasma was collected at 30 min after injection, and glucose was determined (red bars). Hypothalami were collected at 1 h, and malonyl-CoA concentration was determined (blue bars) (n = 4 per group). (C) Mice deprived of food for 20 h were given glucose at 4 g/kg of body weight at times 0, 1, and 2 h repetitively. Glucose was measured every half-hour, and hypothalamic malonyl-CoA was measured hourly (n = 4 per time point).