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. 2007 Nov 27;104(49):19291–19296. doi: 10.1073/pnas.0709971104

Fig. 2.

Fig. 2.

Seed sites in the 3′ UTRs of Vamp3 and Ctdsp1 are important for miR-124a regulation and Ago2-coimmunoprecipitation. (A) Luciferase activity of sensor constructs bearing the Vamp3 3′UTR with sequential seed site deletions transfected into primary cortical neurons together with the RL expression vector. (B) Sensor constructs containing the Vamp3 and Ctdsp1 3′ UTRs with sequential deletions of the seed sequences were transfected into mouse kidney cells (TCMK1) together with either an expression vector for miR-124a or pcDNA and a vector expressing Renilla luciferase (RL). (C) Sensor constructs containing the Vamp3 and Ctdsp1 3′ UTRs, wild-type or lacking 7-mer seed sites were transfected into 293S(Ago2) cells along with miR-124a or GL3.1 siRNAs. Transcript levels in Ago2-coimmunoprecipitates were measured by RT-QPCR. Error bars represent standard deviation of three independent experiments (*, P ≤ 0.05; **, P ≤ 0.005; Student's t test).