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Validation of targets identified by microarray analysis. 3′ UTRs were cloned into a firefly luciferase sensor construct and transfected into 293S(Ago2) cells along with a Renilla luciferase control and miR-124a or GFP siRNAs. Error bars represent 1 SD. (left 4 genes) Targets that are down-regulated in total mRNA levels. (right 21 genes) Targets that are not significantly down-regulated in total mRNA levels.
