Table 1.
Absorption spectra parameters and lifetimes
| Protein/band | νCO, cm−1* | FWHM, cm−1 | T1, ps |
|---|---|---|---|
| wt-Ngb/N3† | 1,933.4 (84) | 12.1 | 19.3 |
| red-Ngb/N3 | 1,933.2 (80) | 12.4 | 16.0 |
| 3cs-Ngb/N3 | 1,932.7 (51) | 11.1 | 15.9 |
| wt-Ngb/N′0 and N0″† | 1,968.7 (16) | 9.8 | 18.4 |
| red-Ngb/N′0 and N0″ | 1,967.3 (20) | 12.4 | 16.1 |
| 3cs-Ngb/N′0 | 1,954.2 (5) | 6.4 | – |
| 3cs-Ngb/N0″ | 1,964.7 (44) | 14.6 | 16.0 |
wt, wild type; red, disulfide bond reduced; 3cs, disulfide bond removed by mutation.
*The values in parentheses indicate the populations of conformers.
†From ref. 28.