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FFCF parameters
| Protein/band | T2*, ps | Δ1, cm−1 | τ1, ps | Δ2, cm−1 | τ2, ps | Δs, cm−1 |
|---|---|---|---|---|---|---|
| wt-Ngb/N3* | 4.4 | 1.9 | 2.0 | 2.7 | 14 | 3.1 |
| red-Ngb/N3 | 6.0 | 3.0 | 1.3 | 3.3 | 22 | 2.9 |
| 3cs-Ngb/N3 | 6.0 | 2.0 | 0.7 | 3.0 | 23 | 2.3 |
| wt-Ngb/N0* | 9.0 | 1.8 | 11.5 | – | – | 3.5 |
| red-Ngb/N0 | 11.0 | 3.0 | 3.7 | – | – | 4.3 |
| 3cs-Ngb/N0 | 6.0 | 2.8 | 8.1 | – | – | 5.6 |
wt, wild type; red, disulfide bond reduced; 3cs, disulfide bond removed by mutation
*From ref. 28.
