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. 2007 Nov 27;104(49):19327–19332. doi: 10.1073/pnas.0705953104

Fig. 8.

Fig. 8.

MMP-7 is required for Notch activation in acinar explants. (A–C) Acinar explants immunostained for Cleaved N1 Val-1744 (green) and amylase (red) on day 3. Wild-type acinar cells exhibited Notch cleavage (A), whereas MMP-7−/− cells did not (B). Inclusion of 200 ng/ml rMMP-7 rescued Notch cleavage in MMP-7−/− acinar cells (C). (Scale bar, 50 μm.) DAPI is shown in blue. (D) RT-PCR for hes1 and hey1 showed increased Notch activation in wild-type acinar cells and MMP-7−/− cells treated with rMMP-7, relative to MMP-7−/− cells. Data are representative of five independent experiments.