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. 2007 Nov 26;104(49):19464–19469. doi: 10.1073/pnas.0703747104

Fig. 2.

Fig. 2.

HSPGs mediate attachment of HIV-1 to DCs. (A) DCs were analyzed for HS expression by FACS. Open histograms represent isotype control; filled histograms represent specific antibody staining. (B) DCs were treated with trypsin and stained for DC-SIGN (DCN46) and HS (10E4). Expression is depicted as mean fluorescence intensity (MFI). (C) DCs were pretreated or not with heparinase I, exposed to 1 ng of p24 HIV-1 for 2 h at 37°C, washed, and lysed, and the p24 content was measured by ELISA. Results are expressed as percentage of p24 captured relative to the inoculum. Error bars represent the standard deviation of duplicates. (D) DCs as well as parental-, syndecan-2-, and syndecan-3-transfected Namalwa cells were treated with heparinase I, II, and III and stained for HS. (E) DCs were pretreated with heparinase III, mannan, or a combination of both; exposed to 1 ng of p24 HIV-1 for 2 h at 37°C; washed; and lysed; and the p24 content was measured by ELISA. Results are expressed as percentage of p24 captured relative to the inoculum. Error bars represent the standard deviation of duplicates (n = 3 donors).