Fig. 4.

Protein profiles of Triton X-100 insoluble fractions treated with various concentrations of Triton X-100. Fractions were subjected to SDS/PAGE and stained by Coomassie brilliant blue (CBB). (Left and Right) Gel images of 5.5% and 12.5% polyacrylamide, respectively. The amount of fraction applied to each lane was adjusted to derive from the same amount of mycoplasma culture. Twice the amount was applied to the 5.5% polyacrylamide gel than that to the 12.5% gel. The molecular mass and the band positions of gliding proteins previously identified are indicated on the left (20). Lane 1, the whole-cell lysate. Insoluble fractions after treatment by 0.01%, 0.03%, 0.1%, and 0.3% Triton X-100 are shown by lanes 2, 3, 4, and 5, respectively. Solid triangles marked a–j indicate protein bands that were identified by PMF. The a′ band is derived from the same protein as that of a. Open triangles indicate protein bands that could not be identified.