Table 1.

Cell counts of individual Fru^M-expressing neuron clusters in 48-h-old pupae by using selective GAL4 driver lines

Group	Cell cluster	Canton-S (M)	l(3)31-GAL4/UAS-traIR (F)	1407-GAL4/UAS-traIR (F)	c1003-GAL4/UAS-traIR (F)	60IIA-GAL4/UAS-traIR (F)	227-GAL4/UAS-traIR (F)
1	fru-mAL	23 ± 2	25 ± 3	23 ± 5	17 ± 4	9 ± 4	0
	fru-PrMs	73 ± 7	68 ± 6	64 ± 6	54 ± 6	33 ± 3	43 ± 6
2	fru-aSP2	42 ± 5	37 ± 6	38 ± 5	26 ± 6	12 ± 2	1 ± 1
	fru-mcAL	31 ± 1	23 ± 4	25 ± 3	16 ± 4	12 ± 2	4 ± 2
3	fru-AL	52 ± 6	40 ± 3	45 ± 8	37 ± 5	39 ± 5	31 ± 6
	fru-P	56 ± 7	31 ± 5	32 ± 7	31 ± 5	38 ± 8	20 ± 4
	fru-MsMt	38 ± 8	30 ± 4	33 ± 4	28 ± 6	18 ± 2	27 ± 6
4	fru-aSP1	12 ± 2	12 ± 2	11 ± 3	11 ± 3	10 ± 2	0
	fru-aSP3	29 ± 5	31 ± 4	31 ± 3	24 ± 2	28 ± 5	18 ± 4
	fru-SG	11 ± 2	3 ± 1	6 ± 2	1 ± 1	2 ± 1	1 ± 1
	fru-pSP1	9 ± 2	3 ± 2	5 ± 2	3 ± 2	4 ± 1	1 ± 1
	fru-pSP2	18 ± 3	16 ± 1	15 ± 1	12 ± 2	13 ± 2	2 ± 3
	fru-pL	12 ± 2	10 ± 1	8 ± 4	5 ± 3	6 ± 3	1 ± 1
	fru-Pr	17 ± 3	16 ± 2	13 ± 2	12 ± 2	8 ± 2	10 ± 2
	fru-MtAb + AB	139 ± 14	40 ± 5	46 ± 4	47 ± 8	38 ± 4	37 ± 3
5	fru-Lv	17 ± 3	16 ± 2	15 ± 2	18 ± 3	17 ± 3	16 ± 1
	Fighting pattern	Male	Male	Intermediate	Female	Female	Female
	Courtship	+	+	+	+	+	+

Brains of masculinized female flies of each of the different lines were stained with Fru^M antibodies, and the numbers of cells in each Fru^M expressing cluster were counted. Shown is the average number of Fru^M-positive cells in each neuron cluster ± standard deviation (N = 10). Groups highlighted in bold are Fru^M clusters that show significant differences in cell number (one-way ANOVA, P < 0.01) between masculinized females and feminized males (see Fig. 1 and SI Table 3). mAL, above the medial antennal lobe; PrMs, between pro- and mesothoracic ganglia; aSP2, anterior region of superior protocerebrum; mcAL, anterior to mechanosensory neuropile of the antennal lobe; AL, cells above antennal lobe; P, posterior region; MsMt, between meso- and metathoracic ganglia; see ref. 22 for other clusters. M, male; F, female.