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Nrf2 nuclear translocation was not blocked by ATRA. Nuclear extracts were prepared from AREc32 cells treated with tBHQ (10 μM), ATRA (1 μM), or tBHQ (10 μM) plus ATRA (1 μM) for 24 h. Nuclear protein (20 μg) was separated on 7% SDS/PAGE and Nrf2 quantified by Western blotting. Data are representative of three separate experiments.
