Fig. 2.

Specific loss of chitin-induced ROS generation in cerk1 mutants and the recovery in the transformants complemented with the WT CERK1 gene. (A) ROS generation by cerk1-1 and cerk1-2 mutants treated with 100 μg·ml⁻¹ of (GlcNAc)₈. ROS was analyzed at 0, 30, 60, and 120 min after the elicitor treatment (shown in white, stripe, gray, and black, respectively). WT, WT A. thaliana; GN8, N-acethylchitooctaose, DW, control seedlings treated with water. (B) ROS generation by cerk1-1 and cerk1-2 mutants treated with 100 μg·ml⁻¹ of P. aeruginosa LPS. (C) Recovery of CERK1 expression in the cerk1-1 mutants complemented with WT CERK1 gene. (D) Recovery of chitin-induced ROS generation in the same transformants.