Table 1.

Vitamin B1 content of WT and thiC mutant plants

Sample	Vitamin B1, ng mg−1 FW	% of WT
WT	0.47 ± 0.04	100
thiC	0.14 ± 0.05	30

Total vitamin B1 content was quantified in whole seedlings employing a microbiological assay. Seedlings were grown in sterile culture in medium lacking vitamin B1 and analyzed 10 days after germination. The results shown are the average of four independent experiments. In the case of the thiC mutant, segregating homozygous seedlings from a heterozygous population of line 17 were used.