Table 2.
Relative metabolite levels between WT and thiC
| WT − B1 | thiC − B1 | thiC + B1 | |
|---|---|---|---|
| Amino acids | |||
| Alanine | 1.000 ± 0.053 | 9.659 ± 0.913 | 1.448 ± 0.053 |
| Arginine | 1.000 ± 0.080 | 1.283 ± 0.110 | 2.458 ± 0.144 |
| Asparagine | 1.000 ± 0.070 | 6.316 ± 0.534 | 5.037 ± 0.248 |
| Aspartate | 1.000 ± 0.042 | 0.964 ± 0.025 | 1.098 ± 0.006 |
| β-alanine | 1.000 ± 0.027 | 3.545 ± 0.155 | 1.396 ± 0.019 |
| Cysteine | 1.000 ± 0.043 | 3.513 ± 0.123 | 4.329 ± 0.135 |
| Glutamate | 1.000 ± 0.050 | 3.018 ± 0.128 | 1.416 ± 0.054 |
| Glycine | 1.000 ± 0.255 | 0.131 ± 0.009 | 0.051 ± 0.003 |
| Lysine | 1.000 ± 0.013 | 4.563 ± 0.243 | 4.392 ± 0.101 |
| Methionine | 1.000 ± 0.027 | 8.292 ± 0.598 | 8.103 ± 0.290 |
| Phenylalanine | 1.000 ± 0.071 | 23.397 ± 1.129 | 16.529 ± 0.514 |
| Proline | 1.000 ± 0.046 | 1.216 ± 0.029 | 2.119 ± 0.088 |
| Serine | 1.000 ± 0.022 | 1.285 ± 0.014 | 0.539 ± 0.005 |
| Threonine | 1.000 ± 0.008 | 1.290 ± 0.023 | 0.575 ± 0.005 |
| Tryptophan | 1.000 ± 0.142 | 44.130 ± 4.096 | 42.691 ± 1.915 |
| Tyramine | 1.000 ± 0.038 | 1.129 ± 0.023 | 1.061 ± 0.028 |
| Valine | 1.000 ± 0.048 | 2.840 ± 0.159 | 1.980 ± 0.040 |
| Organic acids | |||
| Butyrate | 1.000 ± 0.057 | 6.111 ± 0.347 | 2.479 ± 0.045 |
| Citrate | 1.000 ± 0.144 | 1.211 ± 0.172 | 0.835 ± 0.122 |
| Fumarate | 1.000 ± 0.054 | 0.231 ± 0.016 | 0.220 ± 0.005 |
| Glycerate | 1.000 ± 0.107 | 1.309 ± 0.094 | 0.526 ± 0.033 |
| Glycerol | 1.000 ± 0.064 | 0.693 ± 0.031 | 0.690 ± 0.089 |
| Isocitrate | 1.000 ± 0.078 | 1.769 ± 0.134 | 1.704 ± 0.040 |
| Malate | 1.000 ± 0.058 | 2.509 ± 0.271 | 0.938 ± 0.033 |
| Sugars | |||
| Fructose | 1.000 ± 0.078 | 1.443 ± 0.066 | 1.412 ± 0.121 |
| Gentiobiose | 1.000 ± 0.085 | 0.196 ± 0.031 | 0.435 ± 0.006 |
| Glucose | 1.000 ± 0.019 | 1.681 ± 0.128 | 1.801 ± 0.201 |
| Raffinose | 1.000 ± 0.154 | 0.453 ± 0.041 | 0.382 ± 0.016 |
| Ribose | 1.000 ± 0.022 | 0.293 ± 0.012 | 0.373 ± 0.017 |
| Sucrose | 1.000 ± 0.023 | 0.789 ± 0.036 | 0.782 ± 0.046 |
| Trehalose | 1.000 ± 0.023 | 1.031 ± 0.047 | 0.718 ± 0.011 |
Samples were grown in the absence or presence of thiamin (B1) (0.5 μM) as indicated and cotyledons were harvested 11 days after germination. Substantial increases are shown in bold (≥2-fold), whereas decreases (≤0.5-fold) are italicized. Standard errors are the average of six experimental repetitions. Quantitative data are provided in SI Table 4.