Skip to main content
. 2007 Dec 3;104(50):19989–19994. doi: 10.1073/pnas.0708725104

Fig. 4.

Fig. 4.

Colocalization of the retained C18R PPTH and the up-regulated BiP. (A) The cells transfected with either wild-type or C18R PPTH cDNA were coimmunostained with anti-PTH and anti-BiP primary antibodies, followed by secondary antibodies conjugated with Alexa Fluor 488 (green) or Alexa Fluor 546 (red), respectively. (Left and Center) The slides were visualized for DAPI (blue) and PPTH (green) and BiP (red). (Right) A merged image is shown. In C18R PPTH-transfected cells, note the more intense staining of BiP and its colocalization with the hormone (yellow spots). (B) Lysate prepared from wild-type (lane 1) or C18R PPTH (lane 2) cDNA-transfected cells were subjected to Western blot analysis (20 μg protein per lane) with anti-BiP primary and HRP-conjugated secondary antibody. (C) Densitometry analysis of the blot showing 5-fold up-regulation of BiP in C18R PPTH-expressing cells.