Fig. 5.

Up-regulation of the UPR markers (PERK and CHOP) in cells expressing C18R PPTH. (A) In a separate set of experiments, the status of PERK and CHOP were analyzed immunohistochemically in cells transfected with wild-type or C18R PPTH cDNA by using respective primary and appropriately labeled secondary antibodies. The slides were visualized for DAPI (blue), PPTH (green), and PERK/CHOP (red). Up-regulation of both the markers is clearly visible in C18R PPTH-expressing cells. (B) Quantification of the results by counting cells positive for PERK or CHOP from three fields of different experimental setups. Values expressed as percentage of cells positive for the presence of the hormone (gray bar, wild-type; black bar, C18R PPTH).