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. 2007 Dec 6;104(50):20001–20006. doi: 10.1073/pnas.0709986104

Fig. 5.

Fig. 5.

Effects of JJAZ1 and JAZF1–JJAZ1 transgenes on 293 cells with and without knock down of endogenous JJAZl expression. (A) 293 cells that were infected previously with virus encoding siRNA targeting JJAZ1 mRNA 5′ of the fusion point (419-JJAZ1) were infected again with viral vectors expressing JJAZ1 (MIGRI-JJAZ1), JAZF1–JJAZ1 (MIGRI-J-J), or no insert (MIGRI-empty). Histone 3 served as loading control. Compared with empty vector control (MIGRI-empty), JAZF1–JJAZ1 reestablished EZH2 and H3K27 trimethylation to a level similar to that in parental 293 cells. (B) Effect of JAZF1–JJAZ1 on 293 cell proliferation after siRNA knockdown of JJAZ1 expression. Introducing JAZF1–JJAZ1 induced faster growth rate than parental 293 cell. (C) Effect of JAZF1–JJAZ1 on 293 cell proliferation without siRNA knockdown of JJAZ1 expression. JAZF1–JJAZ1 expression reduced the cell proliferation rate compared with empty vector control.